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KMID : 1101220010330020217
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2001 Volume.33 No. 2 p.217 ~ p.228
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The Investigation on AgNOR and PCNA in Normal and Follicular Neoplasia of Thyroid Tissue
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Kim Tai-Jeon
Han Kyung-Hee
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Abstract
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The histologic or cytologic distinctions between follicular adenoma and carcinoma in thyroid tissue are sometimes difficult. For the diagnosis of follicular carcinoma, invasion of blood vesseles or capsules are considered defmitive criteria. However, the distinction of follicular adenoma from carcinoma may be difficult when these features are absent. Furthermore, in case with fme needle aspiration biopsy specimen, the distinction between follicular adenoma and carcinoma may also be difficult, as both are characterized by follicular form cells. The authors performed the sliver nucleolar organizer region(AgNOR) technique and immunohistochemistry for proliferating cell nuclear antigen(PCNA) on 5 samples of normal thyroid tissue, 8 follicular adenomas, and 10 carcinomas to evaluate whether these techniques can aid in these differential diagnosis. Significant differences were found in the mean AgNOR count between normal thyroid tissue (1.29±0.65) and follicular adenomas (2.88±1.33) (P<0.0001), but not between follicular adenomas and carcinomas(2.71±1.14). In the normal thyroid tissue, AgNORs were smooth and round ; in the follicular adenomas and carcinomas, however, they varied in size and shape. Compound AgNORs were commonly present in the tumors but were absent in normals. Antibody to PCNA did not stain normal thyroid tissue cells but stained many follicular adenomas and carcinoma cells. These results suggested that the AgNOR technique and PCNA immunohistochemistry could be used to distinguish normal thyroid tissue form follicular adenoma, but not distinguish follicular adenorna from carcinoma.
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KEYWORD
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AgNOR technique, PCNA immunohistochemistry, Follicular adenoma, Follicular carcinoma
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